Controlling Pests and Diseases viaGenetic Transformation ...

Controlling Pests and Diseases viaGenetic Transformation ...

Figure 1. Development of golden mosaic disease in the common bean lines engineered for resistance to Bean golden mosaic virus mediated RNAi (T), compared to nontransgenic varieties (NT). Two transgenic lines presented similar resistance curves. Plants were cultivated in the field in

2007 and 2008. Disease evaluation was done visually by recording the first date of vein clearing. Rice Tungro Disease From IRRI Resistance to Cassava Viruses for Africa

Resistance against cassava Geminiviruses via the G5 protein and siRNAs; CP siRNA for a potyvirus (cassava brown streak) CsVMV promoter 35S promoter siRNA siRNA siRNA

against ACMV against EACMV against CBSV PTGS -mediated G5 protein

against ACMV & EACMV protein -mediated Improving the Human Condition through Plant Science Progress in developing transgenic plants of farmer-preferred cassava cultivars Cultivars selected based on farmer input in West and East Africa To date 8 farmer preferred cultivars produce transgenic tissues 5 cultivars yielded transgenic plantlets (to date), examples below:

Transgenic plant of Ugandan cv. Ebwanateraka GFP expressing roots & leaves Transgenic plant of Nigerian cv. TME117 GFP expressing roots Improving the Human Condition through Plant Science

Cassava Field Trials Kenya The VIRCA confined field test (CFT) application is currently at the IBC stage. This is being split into two applications. One for each technical approach. The BioCassava Plus (BC+) CFT is currently at the IBC stage. The second version of the application was resubmitted to the IBC in March.

Uganda The VIRCA CFT was approved by the NBC in January. The import permit was applied for in early April and is expected during May. Best case scenario is planting in August. Improving the Human Condition through Plant Science Uganda, NaCRRI Infrastructure Improvements

CFT Site Biosafety Level II Screenhouse Tissue Culture Facility Advances in Controlling Insects: Beyond Cotton and Maize Insect resistant cowpea Insect resistant brinjal/eggplant Insect resistant potato

Improving the Human Condition through Plant Science Bt brinjal Common Vegetable Most popular vegetable in India after potato Meaty texture, so staple in vegetarian diets 25 calories per serving, with no fat! Grown in all vegetable-growing zones in India but

prominently in 8 States: Andhra Pradesh, Bihar, Karnataka, Maharashtra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal 25 - 80 rounds of pesticides sprayed on brinjal crop during each growing season IIVR IIVR Varanasi Varanasi

Benefits to farmers Bt Non-Bt Non-Bt Cash benefit of Rs 16,000-19,000 per acre and Rs 2,000 crore to our nation

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