Canine Be Bug Dection - University of Florida

Canine Be Bug Dection - University of Florida

Canine Bed Bug Detection: Philip G. Koehler Margie & Dempsey Sapp Endowed Professor of Urban Pest Management University of Florida Department of Entomology and Nematology Building 970 Gainesville, FL. 332611 Bed Bug Detection

Visual detection difficult Early infestations go unnoticed Early control more likely to succeed, and these infestations are less likely to spread and are cheaper to control 15 bed bug nymphs in slots of drywall screw

Bed Bug Infestations Active Live bed bugs Eggs Inactive Dead bed bugs Blood spots Fecal stains Cast skins

Training Materials for a Detector Dogs (US Customs) Training Steps Basic Retrieve Controlled Retrieve

Buried Hides Food Reward Station for Evaluating Canine Scent Detection PVC Cap with Hole Bed Bugs Inside Pill Cup with Perforated Lid

PVC Tube Objectives Determine whether a dog can be trained to detect the scent of Cimex lectularius with a method giving food as a positive reward Determine whether dogs can differentiate between: -Other general household pests -Active from an inactive infestation

Determine if dogs can locate bed bugs in hotel rooms Materials and Methods Scent Vials Materials and Methods Scent-detection stations PVC Cap with Hole

PVC Tube Scent Vial Data Positive indications False positive indications IACUC approval # E732

General Household Pest Experiment 4 dogs, 20 replicates per dog Ants Roaches ~ 1 meter Blank

Termites 5 Live Bed Bugs Bed Bug Detecting Canine Differentiation Between General Household Pests 100

% Indication 80 60 97.5 40 20 0 Bed Bugs

0 0 0 0 Ants Roaches

Blank Termites Bed Bug Materials Experiment 3 dogs, 20 replicates per dog Dead Bed Bugs Feces

~ 1 meter Cast Skins Live Bed Bugs Viable Eggs

Live Insects Blank Differentiation Between Bed Bug Materials 100 % Indication 80

60 100 40 90 20 0 Live Bed Bugs

Viable Eggs 3.33 0 0 0

Feces Dead Cast Skins Blank Hotel Field Experiment 3 vials male adults only- 1, 5, and 10

3 vials female adults only- 1, 5, and 10 2 hotel rooms: One with only female vials One with only male vials Randomly hidden in 17 possible locations 3 dogs, 6 replicates per dog Ability to Locate Bed Bugs in Hotel Rooms 100

% In d ic a tio n 80 60 100 40 88.9

100 100 100 100 20 Vials Containing Females

Vials Containing Males 0 1 5 10 1

5 10 Conclusions Dogs can be trained to detect the scent of Cimex lectularius Dogs differentiated Bed bugs from other household pests Active from inactive infestations

Determined that dogs could locate bed bugs hotel rooms Using a Bed Bug Dog

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